Journal: Molecular Therapy Oncology

Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

doi: 10.1016/j.omton.2026.201185

Figure Lengend Snippet: Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

Article Snippet: The following antibodies were used: FITC anti-mouse F4/80 (clone CI: A3-1), APC anti-mouse CD86 (clone GL-1), FITC anti-mouse CD3 (clone 17A2), APC anti-mouse CD4 (clone GK1.5), and APC anti-mouse CD8 (clone YTS-169), all purchased from Elabscience Biotechnology Co., Ltd.

Techniques: Flow Cytometry

Journal: bioRxiv

Article Title: PREMATURE BIRTH AND CESAREAN SECTION AFFECT NEONATAL CD4 + T CELL GENE EXPRESSION AND CELLULAR FUNCTION

doi: 10.64898/2026.02.21.707199

Figure Lengend Snippet: (A) Principal component analysis of CD4+ T cells from neonates born by natural birth (NB) or cesarean section (CS) at term, and preterm (PT). (B) Volcano plot showing the differentially expressed genes between natural birth and cesarean section samples. (C) Pathway enrichment by overrepresentation analysis with clusterProfiler between natural birth and cesarean section samples. (D) Network of genes and pathways of the natural birth condition.

Article Snippet: The purity of our populations of CD4 + T cells was evaluated with antibodies (anti-CD4 (20-0048-T100) and anti-CD3 (20-0037-T100); TONBO biosciences).

Techniques:

Journal: bioRxiv

Article Title: PREMATURE BIRTH AND CESAREAN SECTION AFFECT NEONATAL CD4 + T CELL GENE EXPRESSION AND CELLULAR FUNCTION

doi: 10.64898/2026.02.21.707199

Figure Lengend Snippet: Gene set enrichment analysis of CD4+ T cells from neonates born by natural birth (NB) or cesarean section (CS) using the GO Biological Processes (A) and KEGG (B) databases. Gene set enrichment analysis of CD4+ T cells from neonates born at term by cesarean section (CS) or preterm, using the GO Biological Processes (C) and KEGG (D) databases. Protein–protein interaction (PPI) network of differentially expressed genes in CD4+ T cells from NB and CS neonates constructed using the STRING database and annotated with the most representative GO Biological Processes terms (E) .

Article Snippet: The purity of our populations of CD4 + T cells was evaluated with antibodies (anti-CD4 (20-0048-T100) and anti-CD3 (20-0037-T100); TONBO biosciences).

Techniques: Construct

Journal: bioRxiv

Article Title: PREMATURE BIRTH AND CESAREAN SECTION AFFECT NEONATAL CD4 + T CELL GENE EXPRESSION AND CELLULAR FUNCTION

doi: 10.64898/2026.02.21.707199

Figure Lengend Snippet: CBMCs from term and preterm neonates were stained with CFSE and either left unstimulated or stimulated by crosslinking CD3 and CD28 molecules for 96 hours, followed by staining with anti-CD4–PerCP-Cy5.5. (A) Representative histograms of CFSE dilution in vaginal delivery, cesarean section, and preterm birth samples; (B) Percentage of division in unstimulated or stimulated CD4+ T cells. Statistical comparisons were performed using the Wilcoxon test for paired samples and the Mann–Whitney U test for unpaired samples. * P < 0.05. The sample sizes were as follows: Natural birth = 5, Cesarean = 6, Preterm = 5.

Article Snippet: The purity of our populations of CD4 + T cells was evaluated with antibodies (anti-CD4 (20-0048-T100) and anti-CD3 (20-0037-T100); TONBO biosciences).

Techniques: Staining, MANN-WHITNEY